ER体内定居的米洛酶和托专门的代谢调节根系微生物群组合
Arpan Kumar Basak1,2,3, Anna Piasecka4, Jana Hucklenbroich3
1Institute of Environmental Sciences, Faculty of Biology, Jagiellonian University, Krakow, 30-387, Poland.
The New phytologist
|September 29, 2023
概括
植物根内质网膜 (ER) 机体及其米洛酶酶塑造了土壤微生物群. 这些结构通过改变根排液来影响根-微生物相互作用,影响植物健康和土壤生态.
科学领域:
- 植物生物学 植物生物学
- 微生物学 微生物学
- 植物与微生物的相互作用
背景情况:
- 细胞内网 (ER) 体是来自ER的专门植物结构.
- ER体中含有PYK10氨酶,这种酶可以分解托衍生的醇糖酸盐 (IGs).
- IGs在植物根微生物相互作用中起着已知的作用.
研究的目的:
- 研究植物根中的ER体在调解与土壤传播微生物相互作用中的作用.
- 了解ER体及其酶含量如何影响根微生物群.
主要方法:
- 使用了缺乏ER体 (nai1) 的突变体,pyk10bglu21 (myrosinases),IG生物合成 (myb34/51/122) 和酸代谢 (cyp79b2b3) 的突变体.
- 在自然土壤中对根微生物群进行分析.
- 评估了根排泄物对微生物群落的影响.
- 在单联实验中测试了植物对真菌内细胞的反应.
主要成果:
- 突变物分别在树叶和内层表现出改变的细菌和真菌群落.
- 来自突变者的根排泄物与野生型 (WT) 相比,不同影响了微生物群体概况.
- 真菌内细胞影响了突变的生长,其中一些影响了pyk10bglu21突变.
结论:
- 根ER体及其myrosinases显著调节根排泄物配置文件.
- 这些调制反过来又影响了根系微生物群的相互作用.
- 植物根-土壤微生物组接口的关键调节者是ER机体.
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