热冲击蛋白家族A成员8是膀癌的预后标记:基于实验和机器学习的证据
Yang Liu1, Zhong-Qi Pang1, Jian-She Wang1
1Department of Urinary Surgery, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
Journal of cellular and molecular medicine
|September 29, 2023
概括
热冲击蛋白8成员 (HSPA8) 在膀癌 (BC) 中表达高,与较差的结果相关. 向HSPA8可能为BC患者提供一种新的治疗策略,影响瘤生长和免疫反应.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 热冲击蛋白8成员 (HSPA8) 是真核细胞中非常丰富的伴侣.
- 在膀癌 (BC) 中HSPA8的特定生物作用在很大程度上仍未定义.
研究的目的:
- 研究HSPA8在膀癌 (BC) 进展和预后中的作用.
- 开发基于HSPA8表达的BC的预后模型.
主要方法:
- 在BC细胞系和组织中分析HSPA8表达.
- 功能性测试包括基因淘汰和过度表达.
- 使用 LASSO 考克斯回归的机器学习模型开发.
- 对瘤微环境和免疫细胞透的分析.
主要成果:
- 在BC组织中,HSPA8表达升高,并与高级T阶段和较差的整体存活率 (OS) 相联系.
- 在HSPA8 Knockdown中,它抑制了BC细胞的生长和迁移,同时增加了细胞灭绝.
- 一个机器学习模型将HSPA8确定为一个独立的预后因素,区分具有不同临床结果的高风险和低风险组.
- 高风险组表现出较高的TP53突变率,明显的途径丰富和不利的免疫微环境特征,包括较低的免疫细胞透率和对免疫疗法的反应较差.
结论:
- HSPA8是膀癌预后的一个关键决定因素.
- HSPA8表达水平可以预测患者的结果,并为治疗策略提供信息.
- 针对HSPA8可能是治疗膀癌的有希望的途径,可能调节瘤免疫力.
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