由IL-1β预处理的骨质介质干细胞衍生的外体通过调节SIRT1/ERK通路来减轻败血症内 плазма网膜应激
Xinsheng Cheng1,2, Shikai Wang2, Zhipeng Li2
1Division of Trauma and Acute Care Surgery, Department of Surgery, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu Province, China.
Heliyon
|September 29, 2023
概括
来自IL-1β原始介质干细胞 (IB-exos) 的外体细胞通过减少内分泌网膜应激来防止败血症引起的肺损伤. 这种治疗策略针对SIRT1/ERK通路,为败血症治疗提供了一种新的方法.
科学领域:
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
- 再生医学是一种再生医学.
背景情况:
- 细胞内膜网膜 (ER) 的压力是败血症引起的器官损伤的关键因素.
- 针对ER压力对于开发有效的败血症治疗方法至关重要.
- 由介质干细胞 (MSC) 衍生出来的外体,特别是当用化学激素进行原始化时,显示出治疗潜力.
研究的目的:
- 为了研究从IL-1β预处理的MSC (IB-exos) 中衍生出来的外体的治疗功能.
- 阐明IB-exos在减轻败血性ER压力的基础分子机制.
主要方法:
- 在MSC中,用IL-1β进行了预先条件,以产生IB-exos.
- 实验室和体内毒症模型分别使用HUVECs (LPS诱导) 和小鼠 (CLP诱导) 建立.
- 评估了细胞活力,细胞亡,运动性,管形成,ER压力标志物,SIRT1和ERK酸化.
主要成果:
- IB-exos增强了HUVEC的活力,运动性和管形成,同时减少了亡.
- IB-exos治疗阻断了LPS/CLP诱导的ER压力.
- IB-exos恢复了SIRT1表达和ERK酸化,而SIRT1倒置逆转了这些影响.
结论:
- 通过SIRT1/ERK通路抑制ER压力,IB-exos可缓解败血症引起的肺损伤.
- IB-exos代表了一种有前途的治疗策略,用于减轻与败血症相关的肺损伤.
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