使用冷微光切割断层扫描仪对水和脏解剖结构进行3D自光成像
Guoqing Fan1, Chenyu Jiang1, Zhuoyao Huang1
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Innovation Institute, Huazhong University of Science and Technology, Wuhan 430074, China.
Theranostics
|September 29, 2023
概括
在体内冷固定 (IVCF) 和冷微光切割断层扫描 (cryo-MOST) 允许详细的整个脏成像水缩. 这种方法在小鼠模型中揭示了明显的病理变化,有助于了解疾病.
科学领域:
- 腎病學和泌尿病學.
- 医学成像和诊断 医学成像和诊断
- 病理和疾病机制 病理和疾病机制
背景情况:
- 准确的视觉化整体脏解剖体 in vivo 对于诊断水和了解其原因至关重要.
- 现有的成像技术在生理 perfusion 条件下难以提供整个脏的微米级分辨率.
研究的目的:
- 开发和应用一种新型的成像技术,用于全脏结构的中镜可视化in vivo.
- 量化评估不同小鼠水缩模型中的病理变化.
主要方法:
- 利用体内冷固定 (IVCF) 来保持组织完整性.
- 采用冷微光切割断层扫描 (冷MOST) 与自流光成像用于细胞分辨率可视化.
- 分析了单边尿路阻塞 (UUO) 的脏和db/db小鼠模型的水缩.
主要成果:
- IVCF成功维持了体内脏状况,通过微计算机断层扫描证实了这一点.
- 克里奥-MOST可视化脏结构,包括血管和管道,没有对比剂.
- 在UUO和db/db小鼠中观察到明显的体积和形态变化,UUO显示血管损伤和毛细血管密度降低,表明缺氧,而db/db小鼠表现出膀回流和膀过度活跃作为潜在的原因.
结论:
- 结合IVCF的冷MOST提供了一种强大的工具,用于定量,三维评估水缩中的病理.
- 该方法准确地捕捉了液体分布和结构变化,提供了对疾病机制的洞察力.
- 这种技术有可能用于更广泛的应用,用于用于疾病研究的其他器官和组织的可视化.
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