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Updated: Jul 15, 2025

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
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通过使用SABRE超极化低场NMR研究的生物分子相互作用

Pierce Pham1, Christian Hilty1

  • 1Department of Chemistry, Texas A&M University 3255 TAMU College Station TX 77843 USA chilty@tamu.edu.

Chemical science
|September 29, 2023
PubMed
概括

低场核磁共振 (NMR) 能够使用超极化标签进行生物分子相互作用测量. 这种方法允许在没有强磁体的情况下在近乎生理条件下灵敏地检测连接体结合和蛋白质相互作用.

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Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
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