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与多芳香碳化合物的联可以有利于催化RNAs的活性

Kevin J Sweeney¹, Tommy Le¹, Micaella Z Jorge²

¹Department of Chemistry and Biochemistry, University of California San Diego La Jolla CA 92093 USA ufmuller@ucsd.edu.

Chemical science

|September 29, 2023

概括

短和多芳香碳化合物 (PAHs) 可以增强催化RNA (核糖酶) 的活性. 这一发现为我们提供了关于生命早期的洞察力.

科学领域:

生命的起源研究生命的起源研究
生物化学生化学
RNA世界假设

背景情况:

早期生命可能利用催化RNA (核糖酶) 执行现在由蛋白质执行的功能.
生命的出现涉及复杂的化学混合物,包括氨基酸和.

研究的目的:

调查短是否可以促进催化RNAs的出现和功能.
在生命早期的背景下探索-RNA相互作用.

主要方法:

在实验室中从随机序列中选择催化RNA.
在 prebiotically可信的 (八种氨基酸) 的存在下测试 ribozyme 活性.
评估修饰 (Fmoc组) 和PAH结合的影响.

主要成果:

产生了数十个 ribozymes,其中一个显示 ~ 900 倍的活动增加与特定的.
上N端Fmoc保护组对于增强 ribozyme 活性至关重要.
酸与多芳香碳化合物 (PAHs) 的合物也显著增强了 ribozyme 的功能.

结论:

短,特别是当它与PAHs被修饰或结合时,可以作为 ribozymes 的强有力的辅因子.
这些发现支持-PAH结合体在早期生物系统和生命起源中的潜在作用.

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