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黑色素通过抑制外基因组miR-27b-3p表达来抑制胃癌细胞的增殖

Yi-Qiang Zhang¹, Shuai-Shuai Shi², Yun-Fei Li³

¹Department of Biochemistry, Changzhi Medical College, Changzhi, P.R. China; yiqiangzhang@czmc.edu.cn.

Anticancer research

|September 29, 2023

概括

黑色素治疗降低了胃癌 (GC) 细胞中的外体miR-27b-3p的调节,促进了扩散和入侵. 黑色素通过准miR-27b-3p-ADAMTS5通路来抑制GC.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

黑素表现出超出其在昼夜节律和炎症中的已知作用的抗癌特性.
胃癌 (GC) 的进展受到各种分子机制的影响.
外体微RNAs (miRNAs) 在细胞间通信和癌症发展中起着至关重要的作用.

研究的目的:

为了研究黑激素对胃癌细胞内外体miRNA的影响.
为了确定受黑治疗影响的特定外体miRNA及其在GC进展中的作用.
为了阐明黑激素在胃癌中的治疗潜力.

主要方法:

外体小RNA测序 (sRNA-seq) 用于选以差异表达的外体小RNA在接受黑素治疗的AGS细胞中.
进行细胞计数工具-8 (CCK-8),伤口愈合和穿孔入侵试验,以评估已识别的miRNAs的功能影响.
使用双路西法酶记者基因测试来确定直接的miRNA标.

主要成果:

黑色素治疗导致了AGS细胞中外体miR-27b-3p的显著下调.
过度表达miR-27b-3p模仿增强胃癌细胞的增殖,迁移和入侵.
miR-27b-3p被确定为ADAMTS5的直接标,其上调促进了GC细胞的增殖,入侵和转移.

结论:

黑色素通过调节外体miR-27b-3p-ADAMTS5通路来抑制胃癌的进展.
外体 miR-27b-3p 在胃癌中充当瘤性miRNA.
黑色素显示出作为胃癌患者治疗剂的潜力.

关键词:

亚当斯5 亚当斯5 黑色素是什么黑色素是什么黑色素是什么外基因组是外基因组的组成部分.胃癌胃癌是一种胃癌.在 miR-27b-3p 中.

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