奎尔素对肉中从层次性毛囊发育起来的颗粒状细胞的作用
1Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
British poultry science
|September 29, 2023
概括
Quercetin 是一种生物黄类物质,可以增强母的粒粉细胞活性和孕激素的产生. 这项研究表明,氨酸促进细胞增殖,减少细胞亡,帮助卵泡发育.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 奎尔是一种生物活性生物黄,在毛囊发育过程中对颗粒细胞 (GCs) 的作用基本上尚未研究.
- 了解GCs的监管对于提高家禽的繁殖效率至关重要.
研究的目的:
- 为了研究氨酸对颗粒细胞活性,增殖,亡和孕激素分泌的体外影响.
- 探索氨酸对关键基因和蛋白质表达的影响,与细胞功能和类固醇生成有关.
主要方法:
- 来自等级毛囊的花质细胞在体外用瑞进行了治疗.
- 细胞活力,增殖和亡被评估使用CCK-8,EDU和亡试验.
- 孕激素分泌量通过ELISA测量,基因/蛋白质表达 (PCNA,ERs,StAR,P450scc,3β-HSD,CYP11A1) 通过RT-qPCR和西方斑点分析.
主要成果:
- 奎尔素显著提高了颗粒素细胞的活力和增殖,同时减少了细胞亡.
- 孕激素分泌和PCNA,StAR和CYP11A1 (蛋白质和/或mRNA) 的表达因氨酸显著增加.
- 奎尔提升了ERα mRNA表达的调节,但对ERβ mRNA水平没有显著影响.
结论:
- 奎尔素积极影响颗粒细胞的功能,促进增殖和孕激素的合成.
- 这些发现表明,奎尔素可能是增强卵泡发育和产卵母生殖能力的潜在药物.
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