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整合单细胞和空间转录组学揭示了与内分泌网膜压力相关的CAF亚群,与瘤进展相关
Tao-Lan Zhang1,2, Chao Xia3, Bo-Wen Zheng1,4
1Institute of Clinical Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, China.
Neuro-oncology
|September 29, 2023
概括
瘤中与癌症相关的纤维细胞 (CAFs),特别是那些经历了内分泌网膜应激 (ERS-CAFs) 的纤维细胞,与瘤进展和患者的不良结果有关. 针对这些CAF可能为心脏瘤提供新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 癌症生物学 癌症生物学
- 瘤微环境 瘤微环境
背景情况:
- 冠状瘤的发育和进展受到状层的成分的影响,癌症相关纤维细胞 (CAF) 是一个关键的细胞类型.
- 了解CAFs在瘤中的异质性和作用对于推进治疗策略至关重要.
研究的目的:
- 使用多种omics方法剖析CAFs在chordoma中的异质性,空间分布和临床影响.
- 为了确定与瘤进展和不良预后相关的特定CAF亚群.
主要方法:
- 单细胞RNA测序 (scRNA-seq) 的初级和复发性心脏瘤样本和对照组织.
- 空间转录学,大量RNA-seq分析患者数据,并多重定量免疫光学 (QIF) 用于验证.
- 细胞通信和伪时间轨迹分析,以了解CAF的起源和相互作用.
主要成果:
- 一个独特的CAF亚群,其特点是缺氧基因的高表达和在内质网膜压力 (ERS-CAFs) 中的丰富,在复发性冠状瘤中被确定.
- ERS-CAFs起源于正常纤维细胞,并与瘤和免疫细胞广泛相互作用.
- ERS-CAF签名得分,密度和瘤细胞的接近与瘤入侵,恶性表型和不良患者结果相关.
结论:
- 这项研究阐明了冠状腺瘤中的CAF景观,突出了ERS-CAFs的重要作用.
- 这些发现提供了对瘤病原学的关键见解,并建议ERS-CAF作为潜在的治疗点.
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