流感H7N9病毒破坏了单层人脑的微血管内皮细胞屏障 in vitro
Yuxuan Lei1, Ying Sun2, Weihua Wu2
1School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, 518107, China.
Virology journal
|September 29, 2023
概括
流感H7N9病毒可以感染脑细胞,破坏血脑屏障. 这一发现为H7N9相关的脑损伤和神经复杂症提供了潜在的解释.
科学领域:
- 病毒学 病毒学
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
背景情况:
- 流感H7N9 (H7N9) 感染的死亡率很高,经常导致肺炎.
- 虽然肺炎与细胞因子风暴有关,但H7N9中枢神经系统 (CNS) 并发症的机制尚不清楚.
研究的目的:
- 研究H7N9引起的中枢神经系统并发症的致病机制.
- 为了确定H7N9病毒是否可以破坏血脑屏障 (BBB) 的完整性.
主要方法:
- 利用人类大脑微血管内皮细胞 (hCMEC/D3) 来建模BBB.
- 评估了H7N9病毒感染对BBB完整性和透性的影响.
- 测量了关键细胞连接蛋白的表达:克劳丁-5,奥克卢丁和VE-cadherin.
主要成果:
- 证明H7N9病毒可以感染大脑的微血管内皮细胞.
- 表明H7N9感染损害了BBB的完整性并增加了透性.
- 在H7N9感染后观察到细胞连接蛋白 (claudin-5,ocludin,VE-cadherin) 的下调.
结论:
- 在实验室中,H7N9病毒可以感染并损害血脑屏障的功能.
- 由H7N9感染引起的BBB功能障碍是H7N9病毒脑病变的潜在机制.
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