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在癌症中使用基于CRISPRi的脱屏幕识别必要的长非编码RNA

Oscar Bril¹, Laura J Schwarzmueller¹, Leandro F Moreno¹

¹Center for Experimental and Molecular Medicine, Cancer Center Amsterdam and Amsterdam Gastroenterology Endocrinology Metabolism, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, Amsterdam 1105AZ, The Netherlands; Oncode Institute, Meibergdreef 9, Amsterdam 1105AZ, The Netherlands.

|September 29, 2023

概括

这项研究引入了CRISPR干扰 (CRISPRi) 脱落选协议,以识别癌症中必需的长非编码RNA (lncRNAs). 这种方法系统地发现了对癌症进展至关重要的lncRNAs.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

长非编码RNAs (lncRNAs) 在癌症的发展和进展中起着至关重要的作用.
基于CRISPR的高通量查是功能基因组学的一个强大的工具.

研究的目的:

通过基于CRISPR干扰 (CRISPRi) 的退出查来确定癌症中必不可少的lncRNA的详细方案.
概述了选择目标部位,设计导向RNA和生成功能查的CRISPRi细胞系的方法.

主要方法:

克里斯普尔干扰 (CRISPRi) 技术用于基因沉默.
退学查以确定必要的基因或调控元素.
在癌症细胞系中对长非编码RNA (lncRNA) 功能进行系统评估.

主要成果:

一个基于CRISPRi的脱学查的综合性协议,针对lncRNAs.
选择最佳目标位点和设计有效指导RNA的方法.
为癌症研究生成和利用CRISPRi细胞系的指导方针.

结论:

基于CRISPRi的失学查提供了一种强大的方法来识别癌症中功能关键的lncRNA.
该协议促进了对lncRNAs在瘤发生过程中的系统功能基因组学研究.
提出的方法有助于发现与癌症相关的新 lncRNA 标.

关键词:

生物信息学是一种生物信息学.克里斯普尔是什么意思?克里斯普尔是什么意思?细胞生物学细胞生物学基因组学就是基因组学.分子生物学分子生物学

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...