动态DNA网络引导的定向和直角过渡生物催化层
Yu Ouyang1, Jiantong Dong1, Itamar Willner1
1The Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Journal of the American Chemical Society
|September 29, 2023
概括
这项研究介绍了控制生物催化级联的DNA框架. 这些动态网络可以对酶活性进行精确的按需控制,
科学领域:
- 超分子化学
- 生物催化
- 系统化学
背景情况:
- 立体动态网络 (CDN) 是能够根据外部刺激重新配置的动态分子系统.
- 生物催化提供有效的反应途径,但往往缺乏精确的时间和空间控制.
- 将动态框架与生物催化剂相结合,为开发复杂的响应系统提供了机会.
研究的目的:
- 开发一种使用基于DNA的结构动态网络 (CDN) 控制生物催化级联的新系统.
- 通过燃料驱动的CDN重新配置来证明特定生物催化反应的方向上调或下调的能力.
- 设计一个直角运行的CDN系统,以精确控制生物催化过程,包括纤维素-纤维素转换模型.
主要方法:
- 通过生物催化剂 (葡萄糖氧化酶/胡卜过氧化酶和乳酸脱酶/NAD+) 功能化的CDN组装.
- 使用燃料触发器诱导CDN的短暂重新配置,从而控制绑定的生物催化级联.
- 创建复合CDN并使用直角燃料链来独立控制多个级联.
- 使用动力模型进行计算模拟,以预测和分析瞬态生物催化级联的性能.
主要成果:
- 已证明生物催化级联 (GOx/HRP和LDH/NAD+) 的定向过渡性上调和下调.
- 成功组装复合CDN并实现对单个级联的正交瞬态控制.
- 使用CDN框架表现出调节血栓诱导纤维素纤维素凝固的能力.
- 计算模型在不同的条件下准确地预测了级联性能.
结论:
- CDN框架提供了一个强大的平台,用于创建暂时控制的,短暂的生物催化级联.
- 这些级联的正交触发可以对生物化学过程进行复杂的调节.
- 这种方法为设计响应性分子系统提供了新的策略, 具有在诊断和治疗方面的潜在应用.
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