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多omics分析揭示了肠道微生物组和宿主微RNA在性结肠炎中的相互作用

Lingyan Ma¹, Chenyang Hou¹, Hua Yang¹

¹State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Annals of medicine

|September 29, 2023

概括

这项研究揭示了结肠炎中关键的肠道微生物组和microRNA (miRNA) 变化,突出了它们之间的潜在相互作用. 针对特定的miRNAs可以为炎症性肠病 (IBD) 提供新的治疗策略.

科学领域:

胃肠病学胃肠病学
微生物学微生物学
分子生物学分子生物学

背景情况:

炎症性肠病 (IBD) 是一种与肠道微生物群失调相关的慢性胃肠炎症,病因不明.
微RNA (miRNA) - 微生物群相互作用对宿主健康和疾病至关重要.
了解这些相互作用对于IBD研究至关重要.

研究的目的:

在硫酸德克斯 (DSS) 诱导的性结肠炎 (UC) 模型中调查肠道微生物组和宿主miRNA配置文件.
为了识别与大肠炎相关的微生物和miRNA特征.
探索IBD病变发生过程中的潜在的微生物组-miRNA交叉.

主要方法:

在小鼠中使用了DSS诱导的大肠炎模型.
执行了元基因组测序,代谢组概况,mRNA和miRNA测序.
分析了差异表达的miRNA,基因,细菌生物标记物和代谢物.

主要成果:

在大肠炎小鼠中确定了986个miRNA,其中41个是上调的,21个是下调的.
在免疫和炎症途径中发现了miRNA目标基因的丰富.
在大肠炎中检测到不同的细菌特征 (例如,增加了*Escherichia coli*,*Shigella flexneri*;减少了*Bifidobacterium pseudolongum*) 和改变了代谢物水平 (组胺,N-乙组胺,葡萄糖酸).
揭示了微生物群和结肠miRNA之间的潜在交叉声.

结论:

这项研究阐明了UC期间的多原子关系.
结果表明特定的miRNAs作为IBD的潜在治疗点.
突出了微生物组-miRNA相互作用在IBD发展中的作用.

关键词:

大肠炎是什么意思这是一个微型RNA.我们的肠道微生物群.免疫系统是免疫系统的.代谢物的代谢物.

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