在三重阴性乳腺瘤小鼠模型中,作为向免疫治疗候选人的抗EGFR生物工程细菌外膜囊泡
Razieh Rezaei Adriani1, Seyed Latif Mousavi Gargari2, Hamid Bakherad3
1Department of Biology, Shahed University, Tehran, Iran.
Scientific reports
|September 29, 2023
概括
装有托尔类受体 (TLR) 激动剂的工程外膜囊泡 (OMV) 在克服对癌症免疫疗法的抵抗方面表现有前途. 这些向的纳米颗粒激活瘤微环境中的免疫细胞,增强抗癌反应.
科学领域:
- 生物技术是生物技术.
- 免疫学 免疫学 免疫学
- 纳米医学是一种纳米医学.
背景情况:
- 癌症免疫疗法,特别是检查点抑制剂,面临的挑战是患者无反应率 (55-87%).
- 对于提高非响应性癌症患者的治疗疗效的策略有着至关重要的需求.
研究的目的:
- 开发一种协同的癌症治疗方法,使用携带托尔类受体 (TLR) 激动剂的向纳米粒子.
- 设计生物衍生外膜囊泡 (OMVs) 以在瘤微环境中进行向的输送和免疫激活.
主要方法:
- 外膜囊泡 (OMVs) 是从グラム阴性细菌中设计的,以向过度表达表皮生长因子受体 (EGFR) 的癌细胞.
- 基于亲和力的测试被用来确定目标部分的结合亲和力.
- 在体外和体内研究中,使用EGFR过度表达的癌细胞和三阴性乳腺癌小鼠模型进行了实验.
主要成果:
- 工程OMV在体外表现出对EGFR过度表达的癌细胞的特异性坚持.
- 在体内给药OMV激活了自然杀手细胞,并在瘤微环境中促进了M2到M1巨细胞的两极分化.
- 内和内两种OMV的使用都显示出治疗潜力.
结论:
- 携带TLR激动剂的向OMV代表了增强癌症免疫治疗疗效的有希望的策略.
- 这种方法有效地激活先天免疫细胞并调节瘤微环境.
- OMVs为克服对当前癌症治疗方法的耐药性提供了一个潜在的平台.
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