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Updated: Jul 15, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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Published on: April 30, 2018

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在 Caenorhabditis 线虫杂交体中,有效的针对性与 CRISPR/Cas9 重组,并抑制了重组的发生

Dongying Xie1, Bida Gu2, Yiqing Liu1

  • 1Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China.

BMC biology
|September 29, 2023
PubMed
概括

具有双导向RNA的CRISPR/Cas9能够在物种杂交中实现有针对性的重组 (TR),克服基于同质性的重组 (HR) 抑制. 这一突破有助于在具有挑战性的杂交基因组中进行基因映射,即使进行重新排列.

关键词:
在C.Briggssae的研究中.C. 尼戈尼尼尼尼尼尼尼这就是CRISPR/Cas9的作用.遗传映射 遗传映射是一种基因映射.混合动力是一个混合动力.有针对性的重组组合.

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