一般调节因子7调节先天的免疫信号,以增强棉花中的垂虫枯抵抗力
Fujie Liu1, Sheng Cai1,2, Peng Wu3
1National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, and Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry, Cotton Germplasm Enhancement and Application Engineering Research Center (Ministry of Education), Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China.
Journal of experimental botany
|September 30, 2023
概括
研究人员发现了GauGRF7基因,该基因对植物对菌病原体的免疫力至关重要,例如Verticillium dahliae. 增强GauGRF7增强了植物防御,为作物保护提供了新的策略.
科学领域:
- 植物免疫力 植物免疫力
- 分子植物病理学 分子植物病理学
- 植物的信号传输.
背景情况:
- 植物具有与生俱来的免疫系统,涉及细胞表面和细胞内受体来对抗微生物病原体.
- 实体胚胎发生受体激酶 (SERK) 在各种生理过程中充当共同受体.
- 一般调节因子 (GRF) 蛋白在植物天生的免疫力中的作用基本上是未知的.
研究的目的:
- 研究GRF蛋白在植物天生的免疫信号传递中的功能.
- 为了确定特定的GRF基因参与植物防御的真菌病原体.
- 阐明基因转基因介导植物免疫的分子机制.
主要方法:
- 在对真菌感染的反应中对GauGRF7的基因表达分析.
- 基因沉默和过度表达的研究在棉花和Arabidopsis.
- 对免疫应答标记物的分析,包括辅助NLRs (hNLRs),与病变发生相关的基因,ROS产量和酸水平.
- 在GauGRF7和GhSERK1.1之间进行的蛋白质蛋白相互作用研究.
主要成果:
- 高GRF7在真菌病原体感染时被构成性表达和诱导.
- 沉默GauGRF7会损害植物的免疫力,导致对Verticillium dahliae的易感性.
- 过度表达GauGRF7通过调节ADR1,NRG1和与病变有关的基因来增强对V. dahliae的先天免疫力.
- GauGRF7与GhSERK1相互作用,GhSERK1积极调节了GauGRF7的免疫功能.
- 在转基因系中观察到ROS产量增加和酸水平增加.
结论:
- 在植物对V. dahliae的天生的免疫力中,GauGRF7起着至关重要的作用.
- GRF7-SERK1相互作用对于调节植物免疫反应至关重要.
- 了解GauGRF7的机制为改善作物抵抗真菌病提供了潜在的途径.
相关概念视频
01:28Gene Regulation in Microbial Communities: Quorum Sensing
35
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
35
01:25Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
02:43Plant Cell Wall
56.8K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
56.8K
01:31Regulation of the Unfolded Protein Response
2.5K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K
01:28Global Regulatory Systems
41
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
41
02:04Regulation of Transpiration by Stomata
28.4K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.4K