马皮废弃物作为一种经济原料,用于通过Bacillus circulans生产PHA
Sonika Kag1, Pravir Kumar1, Rashmi Kataria2
1Department of Biotechnology, Delhi Technological University (DTU), Shahbad Daulatpur Village, Bawana Road, Delhi, 110042, India.
Applied biochemistry and biotechnology
|September 30, 2023
概括
马皮废物可以通过微生物发酵将其转化为可生物降解的塑料聚酸盐 (PHA),一种可生物降解的塑料. 这项研究优化了糖的提取和发酵条件,以便从废物中有效地生产PHA.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 废物管理 废物管理
背景情况:
- 聚酸酸盐 (PHA) 是可生物降解和生物相容的聚合物,为传统塑料提供了可持续的替代品.
- 微生物合成PHA发生在压力条件下,碳过多,通常来自石化来源.
- 农业废物的利用,就像土豆皮一样,对于可持续的资源管理至关重要.
研究的目的:
- 调查土豆皮水解盐作为PHA生产的碳来源的潜力.
- 为了优化酸性水解条件,从土豆皮中获得最大的糖分.
- 用土豆皮衍生糖来描述由Bacillus circulans MTCC 8167生产的PHA.使用土豆皮衍生糖.
主要方法:
- 马皮废物的酸性水解,以获得糖水解剂.
- 使用Bacillus circulans MTCC 8167和优化的水解剂进行浸泡发酵.
- 使用富里埃变换红外光谱学,核磁共振和微分扫描热量计的聚合物表征.
- 通过气体染色学和质谱学识别单体.
- 使用克罗托尼酸测定方法量化PHA.
主要成果:
- 优化的酸解产生了最高的糖度在2%的酸,15分钟和121°C.
- 在37°C和36个小时的潜伏期,获得了0.232 ± 0.04 g/L的最大PHA产量.
- 鉴定证实了特定基酸单体的存在.
结论:
- 马皮废弃物是生产聚酸酸盐的可行和可持续原料.
- 优化的发酵条件提高了废物衍生碳来源的PHA产量.
- 这种方法通过将废物转化为有价值的生物塑料,为循环经济做出了贡献.
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