端粒酶逆转录酶通过抑制线粒体超氧化物产生来恢复胰腺微循环概况并减轻内皮功能障碍:急性胰腺炎治疗的潜在标
Xueyan Wang1, Yinan Guo2, Tianyu Cui3
1Department of Internal Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China; Peking University People's Hospital, Beijing 100044, China.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
|September 30, 2023
概括
端粒酶逆转录酶 (TERT) 通过减少有害的超氧化物生产,改善血液流动并减少急性胰腺炎的损伤. 这一发现突出了TERT的特点.
科学领域:
- 生物医学研究的研究.
- 细胞生物学 细胞生物学
- 血管生物学 血管生物学
背景情况:
- 急性胰腺炎 (AP) 涉及严重的微循环功能障碍和内皮损伤.
- 氧化应激,特别是线粒体活性氧物种 (mtROS),加剧了与AP相关的损伤.
- 端粒酶逆转录酶 (TERT) 在维持细胞氧化还原平衡方面具有非正规的作用.
研究的目的:
- 通过抑制超氧化物产生,研究TERT是否可以恢复微循环并减轻AP中的内皮损伤.
- 阐明TERT在AP进展期间内皮功能障碍中的作用.
主要方法:
- 已建立的TERT转基因和敲击AP的小鼠模型使用素 (CER) 和脂多糖 (LPS).
- 使用人类静脉内皮细胞 (HUVECs) 与TERT过度表达或沉默,用LPS刺激.
- 使用流细胞计,共聚焦显微镜和mtROS抑制剂 (MitoTempo) 进行分析.
主要成果:
- 与对照组相比,TERT转基因小鼠显示胰腺微循环和内皮形态有所改善.
- TERT静音减弱了微循环和内皮完整性的恶化.
- 在LPS刺激的HUVEC中,TERT过度表达减少了mtROS的产生和线粒体功能障碍.
- 在内皮功能障碍期间,TERT维持了线粒体含量和ATP水平.
结论:
- TERT有效地恢复了胰腺微循环,并减轻了AP中的内皮病变.
- TERT通过抑制超氧化物产量的增加和减轻线粒体功能障碍来发挥其保护作用.
相关概念视频
01:27Acute Pancreatitis I: Introduction
393
Pancreatitis is inflammation of the pancreas, an organ located behind the stomach. It can be either acute or chronic.
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
393
01:30Acute Pancreatitis II: Clinical Manifestations and Management
142
Acute pancreatitis presents a complex medical emergency characterized by rapid onset inflammation of the pancreas, demanding timely diagnosis and management to prevent complications. The condition primarily manifests through severe upper abdominal pain that often radiates to the back. This pain intensifies following the consumption of fatty foods. Accompanying symptoms such as nausea, vomiting, abdominal distention, fever, dyspnea, cyanosis, and jaundice can vary in intensity but significantly...
142
01:24Chronic Pancreatitis I: Introduction
106
The pancreas, an elongated and flat gland situated behind the stomach, serves a vital function in digesting food and managing blood sugar levels.
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
106
01:29Chronic Pancreatitis II: Collaborative Care
108
The management of chronic pancreatitis is multifaceted, involving a comprehensive approach that includes thorough assessment, diagnostic testing, and a variety of management strategies.
Assessment:
Assessment:
108