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Cristaria plicata 的 GST-Mu 是通过 Nrf2/Keap1 途径调节微囊素的排毒,并具有抗氧化功能

Maolin Feng¹, Yile Hu¹, Lang Yang¹

¹College of Life Science, Education Ministry Key Laboratory of Poyang Lake Environment and Resource Utilization, Nanchang University, Nanchang 330031, China.

Aquatic toxicology (Amsterdam, Netherlands)

|September 30, 2023

概括

这项研究在Cristaria plicata中确定了一种谷氨S转移酶 (GST-Mu),揭示了其抗氧化特性和Nrf2/Keap1通路对微囊等毒素的反应中的调节.

科学领域:

生物化学生化学
环境毒理学环境毒理学
水生生物学水生生物学

背景情况:

谷氨S转移酶 (GST) 是解毒过程中关键的II期酶.
水生生物面临环境毒素,需要了解它们的防御机制.
Nrf2/Keap1通路是抗氧化反应的关键调节者.

研究的目的:

来自Cristaria plicata的mu类GST (CpGST-Mu) 的识别和特征.
在微囊素和过氧化压力下研究Nrf2/Keap1通路对CpGST-Mu表达的调节.
确定CpGST-Mu在赋予抗氧化的作用.

主要方法:

对CpGST-Mu. 的基因鉴定和测序.
亚细胞局部化研究.
酶活性测定 (最佳pH值和温度).
实时定量PCR (RT-qPCR) 用于基因表达分析.
对Nrf2和Keap1.1.进行基因淘汰实验.

主要成果:

CpGST-Mu的cDNA为1026bp,具有558bp的开放读取,局部位于细胞质中.
对CpGST-Mu的最佳活性是在pH 6和40°C时.
CpGST-Mu mRNA的构成性表达,在肝中最高,在中最低.
微囊素和过氧化显著增加了CpGST-Mu mRNA水平.
CpGST-Mu显示对过氧化具有对抗作用.
Knockdown 的 Nrf2 或 Keap1 影响 CpGST-Mu mRNA 水平,表明路径调节.

结论:

CpGST-Mu具有抗氧化特性,并有助于Cristaria plicata中的排毒.
Nrf2/Keap1信号通路调节了CpGST-Mu的表达,以应对氧化应激.
这项研究提供了对软体动物微囊排毒机制的见解.

关键词:

这是一种Cristaria plicata.过氧化是一种过氧化.Mu-类谷氨酸S转移酶:微囊素 Nrf2/Keap1通道中的一个.

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