疾病严重程度损害了从炎症性肠病患者产生肠道器官培养的产生
Wei Ding1, Olivia M Marx2, Marc M Mankarious1
1Division of Colon & Rectal Surgery, Department of Surgery, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, Pennsylvania.
The Journal of surgical research
|September 30, 2023
概括
来自非患有炎症性肠病 (IBD) 组织的器官是成功的. 需要进行进一步的研究,以改善从患有IBD的组织中提取有机物,以研究疾病病理生理学.
科学领域:
- 胃肠病学 胃肠病学
- 细胞生物学 细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 肠表皮的慢性炎症是炎症性肠病 (IBD) 的基础,包括性结肠炎 (UC) 和克罗恩病 (CD).
- 活体肠道有机体作为研究IBD的一个有价值的模型.
- 从手术切除的有病IBD组织中建立有机体的可行性仍然未被充分探索.
研究的目的:
- 评估从无病和有病IBD组织中建立肠道有机体培养的能力.
- 为了在UC和CD患者中比较非患病和患病组织之间的器官衍生成功率.
主要方法:
- 从12名UC和20名CD患者中采集了组织,包括各种结肠区域和CD的末端结核.
- 器官被培养使用Matrigel圆顶和定义的介质.
- 免疫组织化学 (MUC2,Ki67,E-cadherin) 和Alcian蓝色染色在初级组织和有机体上进行.
主要成果:
- 在从未患病的UC组织中确定有机体的成功率高 (92%).
- 患病的UC组织的成功率明显较低,随着疾病的严重程度而下降 (67%轻度,22%中度,4%严重).
- 与非患病的UC组织相比,非患病的CD组织的成功率较低 (48%). 在患病的UC组织中减少的杯细胞和MUC2被保留在有机体中.
结论:
- 肠道器官可以很容易地从非患病的,手术切除的IBD组织中获得.
- 为了有效地从患有IBD的组织中提取有机物,需要进行改进.
- 这项研究支持有机体对调查IBD病理生理学的有用性.
相关概念视频
01:30Inflammatory Bowel Disease II: Crohn's Disease
279
Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
279
01:17Chronic Bowel Disorders: Introduction
474
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
474
01:30Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
348
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
348
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
207
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
207