多omics分析揭示关键途径涉及低C/N比率的压力反应在Pseudomonas sp. 具有更高的去除效率的LW60
Li Wang1, Shipeng Wang2, Chen Chen3
1College of Architecture and Environment, Institute of New Energy and Low-Carbon Technology, Sichuan University, Chengdu, Sichuan 610207, China; Yibin Institute of Industrial Technology, Sichuan University Yibin Park, Section 2, Lingang Ave., Cuiping District, Yibin, Sichuan 644000, China.
Bioresource technology
|September 30, 2023
概括
这项研究分离了Pseudomonas sp. LW60是一种新型细菌,在低温和低C/N比率下有效去除. 它的抵抗机制涉及增强的新陈代谢和运输系统,而不是直接的去除途径.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 废水处理 废水处理
背景情况:
- 在低温和低C/N比率下,废水处理中的去除具有挑战性.
- 现有的研究缺乏对在低C/N比率压力下微生物机制的理解.
研究的目的:
- 在低C/N比率下在低温度下隔离和描述一种细菌菌株,以有效地去除.
- 调查分子机制赋予耐受性低C/N比应力在孤立的菌株.
主要方法:
- 隔离了Pseudomonas sp. 这种病毒. 在4°C的温度下LW60
- 在不同低C/N比率 (2-10) 中评估氨- (NH4+-N) 去除效率.
- 多组体分析 (转录组,蛋白组,代谢组) 以阐明抗性机制.
主要成果:
- 伪omonas sp. 这种病毒是假的. 在4°C和2-10的C/N比率下,LW60表现出高的NH+-N去除效率 (40.02%-100%) .
- 多omics数据表明,对低C/N压力的抵抗与增强的中央碳代谢,氨基酸代谢和ABC载体有关.
- 移除的途径并不是对抗压力的主要贡献者.
结论:
- 伪omonas sp. 这种病毒是假的. LW60是低温,低C/N比率去除的有希望的候选者.
- 该菌株的耐受性机制依赖于代谢和运输系统的适应,为生物工程策略提供了洞察力.
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