通过提高细胞外电子转移能力,提高了优质的阳极电
Liuyan Gu1, Xinxin Xiao2, Sang Yup Lee3
1National Food Institute, Technical University of Denmark, Kongens Lyngby, 2800, Denmark.
Bioresource technology
|September 30, 2023
概括
阳极电发酵 (AEF) 使用阳极作为电子接受器. 一种进化的Lactococcus lactis菌株显示出更快的生长速度,并使用生物电化学系统 (BES) 中的阳极产生了更多的2,3-butanediol.
科学领域:
- 微生物学 微生物学
- 生物电化学 生物电化学
- 代谢工程是代谢工程.
背景情况:
- 阳极电发酵 (AEF) 通过使用阳极作为终端电子接受器,为传统发酵提供了一个有希望的替代方案.
- 为了NAD+再生而设计的Lactococcus lactis菌株可以利用酸,但高度限制了实际使用.
- 适应性进化可以增强生物电化学系统的微生物能力.
研究的目的:
- 研究一种野生型的乳糖菌株和一种通过适应进化 (ALE) 突变的生长和代谢产物,在生物电化学系统 (BES) 中使用阳极作为电子受体.
- 为了比较ALE突变的表现与AEF中的母菌株相比,ALE突变具有增强的酸呼吸能力.
- 评估乳酸细菌在AEF应用中的潜力.
主要方法:
- 使用生物电化学系统 (BES) 设置进行阳极电发酵 (AEF).
- 培养一种野生型的乳球菌株和一种适应性进化 (ALE) 突变物.
- 测量微生物群落产生的生长速度,代谢物产量 (乙,2,3-butanediol) 和电流密度.
主要成果:
- 这两种L. lactis菌株都使用阳极作为电子接受器表现出增长.
- 与母菌株相比,ALE突变体表现出明显更快的生长速度.
- 该ALE突变主要产生2,3-butanediol,而母菌株主要产生乙.
- 该ALE突变实现了0.81±0.05mA/cm2的高电流密度,表明与阳极的有效相互作用.
结论:
- 适应性进化显著提高了Lactococcus lactis在阳极电发酵 (AEF) 中的表现.
- ALE突变体有效利用阳极的能力证明了乳酸细菌在生物电化学应用中的潜力.
- 具有进化菌株的AEF提供了一种可持续的方法来生产价值的化学物质,如2,3-butanediol.
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