来自木材的白色腐烂真菌的特征,该木材已腐烂,因木质素降解而腐烂
Yakath Ali Nurul-Aliyaa1, Nik Azimatolakma Awang2, Masratul Hawa Mohd1
1School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Letters in applied microbiology
|October 1, 2023
概括
这项研究从腐烂的木材中确定了23种白色腐烂真菌 (WRF),评估了它们的素修饰酶 (LME) 生产. 澳大利亚虫 (Phanerochaete australis) 显示出异常的乳糖酶和红素过氧化酶活性,这表明红素降解效率高.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 白色腐烂真菌 (WRF) 对于生态系统中的红素降解至关重要.
- 红素修饰酶 (LMEs),如乳糖酶 (Lac),红素过氧化酶 (LiP) 和过氧化酶 (MnP) 是这一过程的关键.
- 了解WRF多样性和LME生产对于生物技术应用至关重要.
研究的目的:
- 从腐烂的木材中分离和识别WRF.
- 评估这些分离物在各种介质上的LME生产能力.
- 为潜在的应用确定高效的素降解菌株.
主要方法:
- 从腐烂木材样本中分离WRF.
- 使用形态特征和DNA测序 (ITS区域) 进行识别.
- 使用合成染料 (ABTS,蓝色B,红色) 进行Lac,LiP和MnP生产的酶测定.
主要成果:
- 确定了23个属于8个物种的WRF分离物.
- 分离物根据LME生产概况进行分组:16个产生的Lac-LiP-MnP,3个产生的Lac-MnP,3个产生的Lac,1个产生的MnP.
- 南方 (Phanerochaete australis) (BJ38) 的Lac和LiP活性最高,而南方 (Lentinus squarrosulus) (IPS72) 的MnP活性最高.
结论:
- 澳大利亚被确定为一种高效的素降解剂,产生大量的乳糖酶和素过氧化酶.
- 该研究提供了在各种WRF隔离物中LME生产的全面概况.
- 这些发现支持在生物修复和生物质转化过程中选择WRF菌株的潜力.
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