农业工业和食品加工残留物用于固态发酵过程的价值化:优化水解酶联合生产的一个案例
Jazel Doménica Sosa-Martínez1, Julio Montañez1, Juan Carlos Contreras-Esquivel1
1Facultad de Ciencias Químicas. Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo, Coahuila, 25280, Mexico.
Journal of environmental management
|October 1, 2023
概括
农业工业残留物 (AFR) 可以通过固态发酵 (SSF) 转化为有价值的酶. 这项研究优化了从果果汁和酒厂的消耗谷物中产生氨酶,氨酶和细胞酶的条件,从子皮中获得高氨酸回收.
科学领域:
- 生物技术和生物加工
- 可持续的废物管理 可持续的废物管理
- 酶技术 酶技术是一种
背景情况:
- 农业工业和食品加工残留物 (AFR) 的有效管理对于可持续性至关重要.
- 固态发酵 (SSF) 为将AFR转化为增值产品提供了一种可行的方法.
- 来自AFR的真菌酶生产是SSF的一个关键应用.
研究的目的:
- 开发和优化一种系统的方法,通过SSF将AFR转化为有价值的产品.
- 选真菌菌株,并为酶生产选择最佳基质 (果果汁和酒厂的花费谷物).
- 为了确定和优化关键的过程参数,为共合成的西兰酶,乳酶和细胞酶.
主要方法:
- 将AFR作为SSF的基质进行物理化学评估.
- 选五种真菌菌株的酶生产能力.
- 使用普莱克特-伯曼设计和响应表面方法 (中央复合设计) 进行统计优化,以识别和优化关键因素 (BSG/AP比率,pH,湿度).
主要成果:
- 果果汁和酒厂使用的谷物中含有Aspergillus sp. (G5菌株) 被选为最佳基质和菌株.
- 湿度被确定为影响酶共合成的最重要因素.
- 确定了最大限度地提高西兰酶,点酶和细胞酶的最佳条件,使用富含西兰酶的酶提取物产生高点恢复率 (12.33±0.53%).
结论:
- 开发的SSF方法适用于使用AFR的各种生物过程.
- 优化的工艺条件允许量身定制的酶尾酒具有特定的成分.
- 这种方法提供了一种可持续的途径,用于提升AFR的价值,并生产用于工业应用的酶,例如素提取.
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