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修改后的哺乳动物actin异型的净化,用于体外溶解试验

David J Kast1, Silvia Jansen1

  • 1Department of Cell Biology and Physiology, Washington University in St. Louis, Saint Louis, MO, 63110, United States.

European journal of cell biology
|October 1, 2023
PubMed
概括

纯化actin研究已经取得了进展,但早期的方法损害了actin. 较新的,更温和的技术现在产生了本土的actin,用于研究其修饰和疾病突变.

关键词:
在Actin中的Actin是Actin.凝和素是不同的.在N端的乙化.没有8080个.净化 净化 净化 净化在SETD3中,我们可以使用SETD3.

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Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution.  Actin coding genes are conserved within species and across...
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