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子MRE11的分子克隆和功能表征

Jinyue Yang¹, Peng Zhou¹, Wanrong Wu¹

¹State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.

Developmental and comparative immunology

|October 1, 2023

概括

MRE11 (duMRE11) 作为细胞质DNA传感器,对天生的免疫力至关重要. 这项研究描述了duMRE11,揭示了它在激活干扰素生产和对肠炎病毒的抗病毒防御中的作用.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
分子生物学分子生物学

背景情况:

人类MRE11是一种已知的细胞质DNA传感器,参与I型干扰素诱导.
鸟类MRE11在天生的免疫力中的功能在很大程度上仍然未被描述.

研究的目的:

为了第一次克隆和表征MRE11 (duMRE11).
调查duMRE11在子天生的免疫反应和抗病毒防御中的作用.

主要方法:

全长的duMRE11基因克隆和测序.
定量实时PCR用于基因表达分析.
在子胚胎纤维细胞 (DEFs) 中进行过度表达和淘汰研究.
IFN-β促进剂活性测定和肠炎病毒 (DEV) 感染模型.

主要成果:

duMRE11基因编码了一种703氨基酸蛋白质,与的MRE11.11具有很高的相似性.
duMRE11是无处不在的表达,在免疫器官如毛囊,胸腺和脏中的高水平.
duMRE11的过度表达激活了IRF1,NF-κB和IFN-β的产生.
在刺激或DEV感染时,duMRE11倒置会损害IFN-β促进体活性.
duMRE11有效地抑制了DEV的复制.

结论:

子MRE11是一种功能性细胞质DNA传感器,参与天生的免疫力.
duMRE11在诱导子I型干扰素和抗病毒反应方面发挥着重要作用.
这些发现提供了对禽类抗病毒免疫力和潜在治疗点的见解.

关键词:

一个的.如果是IFN-β,那么它是什么?天生的免疫反应.在MRE11中,MRE11是MRE11中的一个.

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