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反氧活性聚纳米颗粒剥夺了内源性谷的电子,用于ROS生成和瘤化学动力学治疗

Yifei Wang¹, Jia Wang², Yunke Jiao¹

¹Key Laboratory for Ultrafine Materials of Ministry of Education, School of Material Science and Engineering, Frontiers Science Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.

Acta biomaterialia

|October 1, 2023

概括

这项研究引入了P(DA-Fc) 纳米颗粒,该纳米颗粒可以将谷氨的作用从ROS清理器转变为ROS生产器,通过产生更具反应性的氧物种来增强癌症治疗的化学动力学疗法 (CDT).

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
在瘤学瘤学.

背景情况:

化学动力学疗法 (CDT) 通过产生活性氧物种 (ROS) 显示出对癌症治疗的希望.
瘤细胞往往没有足够的过氧化 (H2O2) 进行有效的CDT.
氨酸 (GSH) 通常通过清除ROS来保护瘤细胞,从而限制CDT的疗效.

研究的目的:

通过逆转GSH的功能来开发一种新的增强CDT的战略.
使用P(DA-Fc) 纳米颗粒将GSH从ROS吸收器转化为ROS生产器.
通过产生ROS来提高癌症治疗的效率.

主要方法:

制造P(DA-Fc) 纳米颗粒从铁碳酸 (Fc) 合多巴胺.
模仿NOX酶使P(DA-Fc) 能够从GSH中提取电子.
利用P(DA-Fc) 的 - 子转换能力来产生ROS,由Fc触发.
在体外和体内实验来评估ROS产量和抗瘤作用.

主要成果:

P(DA-Fc) 纳米粒子成功地消耗了GSH,并产生了内源的ROS.
ROS生成取决于瘤微环境中的GSH水平和pH值.
在GSH治疗后,P(DA-Fc) 中增强的半基表明了强大的单电子还原能力.
通过产生ROS诱导的缩和亡来证明瘤细胞死亡.

结论:

P(DA-Fc) 纳米粒子有效地扭转了GSH的作用,使其成为增强CDT的ROS生产商.
这一策略克服了瘤中H2O2和GSH介导的ROS清理不足的局限性.
基于PDA的纳米材料在CDT中提供了超越传统应用的新可能性.
这项研究提供了一种新的方法,可以显著提高癌症治疗的CDT效率.

关键词:

电子运输的电子运输.在GSH函数的反向作用下,类似NOX酶的ROS生成器基于多的CDT纳米材料是基于多的.氧化还原活性活动.

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