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改进的病毒度方法,用于清洗水从无透和无透表面的净化污染
Brittany N Hurst1, Asja Korajkic2, Adin Pemberton2
1Department of Environmental Health, University of Cincinnati College of Medicine, 3230 Eden Ave, Cincinnati, OH 45267, USA.
Journal of virological methods
|October 1, 2023
概括
一种使用死胡同空心纤维超过的新方法有效地检测洗水中的病毒. 这有助于评估生物污染物的健康风险,并提高响应者的安全性.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 公共卫生 公共卫生
背景情况:
- 表面消毒使用洗水,但未知使用过的水带来的风险.
- 洗水中的致病微生物,特别是病毒,对健康构成风险.
- 缺乏有效的方法来选洗水中的病毒病原体.
研究的目的:
- 适应死胡同空心纤维超过 (D-HFUF) 用于在洗水中检测病毒.
- 开发一种恢复粒子附着病毒的程序.
- 在模拟的污染清除事件中评估方法的效率.
主要方法:
- 使用模拟的洗水与大肠膜 (体和F+) 被使用.
- 通过和没有沉积物分离比较D-HFUF.
- 经过测试的三豆 (TSB) 和其他缓冲器用于病毒化.
- 在多孔和无孔表面上部署了优化的方法.
主要成果:
- 在过之前分离沉积物显著增加了菌体的回收 (P < 0.0001).
- TSB对体 (108±9%) 和F+大肠膜 (92±9%) 的排泄更有效.
- 优化方法在模拟清除事件中实现了71-96%的细胞恢复.
结论:
- 适应的D-HFUF系统与沉积物分离和TSB化有效检测洗水中的病毒.
- 该方法有助于评估与净化活动相关的健康风险.
- 在洗水中改善病毒病原体的检测,提高了响应者和公共安全.
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