在羊的早期怀孕期间,FABP4调解了内质网膜应激和自,以调节子宫内膜上皮细胞功能
Meijun Song1, Kexing Hao1, Fenghua Qi1
1College of Animal Science and Technology, Shihezi University, Xinjiang 832000, China.
The Journal of reproduction and development
|October 1, 2023
概括
脂肪酸结合蛋白4 (FABP4) 在羊怀孕中起着关键作用. FABP4通过影响子宫内膜网膜应激和早孕期间的自来调节子宫内膜上皮细胞功能.
科学领域:
- 生殖生物学 生殖生物学
- 细胞和分子生物学 细胞和分子生物学
- 生物化学 生物化学
背景情况:
- 动态的子宫内膜变化对于在反动物中早期怀孕的建立至关重要.
- 胚胎囊的延长和植入取决于子宫内膜分泌物.
- 脂肪酸结合蛋白4 (FABP4) 涉及概念体植入,但其在周植入期间在绵羊子宫内皮细胞 (EECs) 中的作用尚不清楚.
研究的目的:
- 调查在围植期期间在绵羊子宫内膜上皮细胞 (SEECs) 中FABP4调节的机制.
- 阐明FABP4在子宫内膜功能,内膜网膜 (ER) 应激和绵羊自中的作用.
主要方法:
- 在羊中模拟体外荷尔蒙变化 (SEECs).
- 使用BMS309403评估了FABP4抑制对SEEC功能,ER压力和自的作用.
- 利用ER压力激活剂和自抑制剂逆转FABP4抑制诱导的变化.
主要成果:
- 激素变化在实验室中提高了SEECs的FABP4表达.
- 抑制FABP4可以减少SEEC迁移,ER压力和自.
- FABP4 抑制剂 BMS309403 抵消了激素诱导的功能变化.
- ER应激激活和自抑制逆转了由FABP4抑制引起的异常前列腺素分泌.
结论:
- 在早孕期间,FABP4调节绵羊子宫内膜功能.
- FABP4影响子宫内膜上皮细胞迁移,ER压力和自.
- FABP4的机制涉及SEECs中ER压力和自途径的调节.
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