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通过基于PACT的被动组织清除来优化骨的光学透明度

Byung-Ho Jin^1,2,3, Jiwon Woo^1,4,5,6, Mirae Lee^1,4,6

¹The Spine and Spinal Cord Institute, Department of Neurosurgery, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, 06273, Republic of Korea.

Experimental & molecular medicine

|October 1, 2023

概括

研究人员开发了Bone-mPACT+,这是一个针对骨的优化组织清理方法. 这种技术提高了透明度,并保持了硬骨组织 (如股骨) 的结构完整性,从而实现了更好的3D分析.

科学领域:

生物医学工程生物医学工程
历史学历史学历史学
显微镜的使用方法

背景情况:

先进的组织清除方法,如被动清除技术 (PACT),使生物结构的3D分析成为可能.
在保持结构完整性和控制硬骨组织在清除过程中的扩张/收缩方面仍然存在挑战.

研究的目的:

提出一个基于PACT的优化骨清除方法,Bone-mPACT+,以提高清除效率和结构保存.
为了评估Bone-mPACT+在不同类型的骨中使用不同的脱程序的疗效.

主要方法:

基于PACT的骨-mPACT+协议的开发和优化.
在小鼠和老鼠的骨头上测试 Bone-mPACT+ 的四种脱过程.
介绍了Bone-mPACT+高级协议,用于处理大而硬的老鼠骨头.

主要成果:

骨-mPACT+显著提高了骨组织清除效率和光学透明度.
几乎所有测试的骨组织,包括小鼠和老鼠的股骨和骨,都变得透明.
通过Bone-mPACT+ Advance协议,可以轻松清除和成像大型,硬的老鼠骨头.

结论:

骨-mPACT+是一种有效的方法,可以清除硬骨组织,同时保持结构完整性.
这种技术扩大了3D组织清理的应用到骨研究中,提高了对骨生物学的理解.
骨-mPACT + 先进协议为具有挑战性的骨样品提供了专门的解决方案.

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