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用于光热增强基因免疫治疗的GSH/pH双响应药物递送系统

Tiantian Ma¹, Wen Li¹, Jingtao Ye¹

¹College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Key Laboratory of Organosilicon Material Technology, Hangzhou 311121, Zhejiang Province, P. R. China. liyang@hznu.edu.cn.

|October 2, 2023

概括

这项研究开发了一种用于乳腺癌治疗的新型纳米载体,将光热疗法与基因沉默结合起来. 该系统有效地提供治疗药物,从而完全根除瘤并增强抗瘤免疫力.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
癌症治疗癌症治疗

背景情况:

乳腺癌是女性死亡的主要原因之一.
光热疗法 (PTT) 对局部瘤有希望,但面临透挑战.
使用小干扰RNA (siPD-L1) 的基因治疗可以通过调节免疫反应来增强PTT.

研究的目的:

开发一种用于向乳腺癌治疗的新型纳米载体.
将PTT与编程死亡配体1 (PD-L1) 的基因沉默结合起来,以获得协同作用的抗瘤效应.
为了克服RNA不稳定性和低最佳药物释放的局限性.

主要方法:

设计了一种对谷氨 (GSH) /pH敏感的菌根系统,封装了绿色氨酸 (ICG) 和siPD-L1.
使用的聚乙烯胺和多P123用于纳米载体配方 (PSP/ICG/siPD-L1).
评估纳米载体的生物相容性,稳定性,药物释放和治疗疗效 in vitro 和 in vivo.

主要成果:

该PSP/ICG/siPD-L1纳米载体显示出良好的生物相容性和稳定性.
观察到ICG和siPD-L1的快速释放是对瘤微环境触发因素 (酸性pH和GSH) 的反应.
siPD-L1降低了PD-L1的表达,而ICG产生了光热效应,从而在体内完成了4T1瘤切除.

结论:

开发的纳米载体系统对于向乳腺癌治疗是有效的.
PTT和siPD-L1交付的协同组合可以增强抗瘤免疫力.
在改善乳腺癌治疗结果方面,PSP/ICG/siPD-L1具有前景.

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