海洋细菌含有硫胺抗性基因sul4,没有移动遗传元素
Suzune Shindoh1, Aya Kadoya1, Reo Kanechi1
1Center for Marine Environmental Studies, Ehime University, Matsuyama, Japan.
Frontiers in microbiology
|October 2, 2023
概括
海洋细菌含有抗生素耐药性基因 (ARG). 该研究发现,硫胺抗性基因sul4在海洋细菌中非常丰富,可能无法水平转移.
科学领域:
- 环境微生物学环境微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 海洋细菌可以作为抗生素耐药性基因 (ARG) 的储存库.
- 海洋环境中的ARG可能来自临床和陆地来源或内在海洋过程.
- 硫胺抗性基因 (sul) 是一种重要的ARG类.
研究的目的:
- 在海洋细菌分离物中调查硫胺耐药基因的流行率和特征.
- 为了确定海洋细菌中最丰富的硫基因.
- 为了确定sul4基因在海洋分离物中的可转移性和潜在功能.
主要方法:
- 74种抗硫胺胺抗性海洋分离物的PCR查检测硫1,硫2,硫3和硫4基因.
- 全基因组测序23个表达硫4的分离物 (α-和γ-蛋白质细菌,细菌).
- 生物信息分析以确定与sul4.4相关的移动遗传元素和基因集群.
主要成果:
- 在选的海洋分离物中,sul4基因是最丰富的硫胺抗性基因 (45%).
- 在33个分离物中检测到硫化物4,明显超过sul2 (6),sul3 (5) 和sul1 (1).
- 全基因组测序显示,sul4与移动遗传元素无关,这表明是克隆转移而不是水平转移. 鉴定出一组叶酸代谢基因,包括作为二甲酸合成酶起作用的sul4.
结论:
- 硫胺4基因在抗硫胺胺的海洋细菌中非常普遍.
- 在这些海洋分离物中,sul4似乎是克隆转移的,而不是水平转移的.
- 硫4可能在海洋物种中的叶酸合成中发挥作用,但在这种情况下不被认为是可转移的ARG.
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