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茶叶杆菌 (Brevibacillus brevis) HNCS-1:一种针对茶叶植物疾病的生物控制细菌

Wenbo Yang^1,2, Hui Yang², Xiaocun Bao²

¹College of Plant Protection, Yunnan Agricultural University, Kunming, China.

Frontiers in microbiology

|October 2, 2023

概括

茶园土壤细菌Brevibacillus brevis HNCS-1有效控制了五种茶叶植物病原体. 基因组分析确定edeine是其主要的抗菌,为生物控制发现提供了一个新的战略.

科学领域:

农业微生物学农业微生物学
细菌遗传学细菌遗传学
生物控制剂生物控制剂

背景情况:

菌细菌是农业中重要的生物控制剂.
抗菌 (AMP) 是具有抗菌性能的Brevibacillus的关键产品.
茶叶疾病对作物产量和质量构成重大威胁.

研究的目的:

为了隔离和描述一种具有对茶病原体的对抗活性的Brevibacillus菌株.
阐明隔离菌株中生物控制机制的遗传基础.
为了确定负责观察到的对抗作用的特定抗菌.

主要方法:

从茶园土壤中分离了Brevibacillus brevis HNCS-1的菌种.
对五种主要茶叶植物病原体进行致病性测试.
全基因组测序和比较基因组分析.
用UHPLC-MS/MS分析检测和识别抗菌.

主要成果:

这种HNCS-1菌株对Gloeosporium theae-sinensis,Elsinoe leucospira,Phyllosticta theaefolia,Fusarium sp.和Cercospora theae.表现出对抗性的作用.
基因组分析揭示了与其他 Br. 共同的核心基因组. 布雷维斯菌株,含有单个非核糖体合成酶 (NRPS) 基因集群用于edeine.
UHPLC-MS/MS证实edeine A和edeine B是HNCS-1菌株产生的主要抗菌.

结论:

埃迪因被确定为Br.生产的主要抗菌. 布雷维斯HNCS-1. 这就是它.
该研究提供了一种有效的策略,用于识别来自其他生物控制细菌的新型抗菌产品.
这项研究增强了对农业中Brevibacillus介导的生物控制机制的理解.

关键词:

布雷维细菌 (Brevibacillus brevis) 是一种细菌.具有对抗性的活动.抗微生物类的抗微生物.埃迪内埃德是什么意思基因组注释基因组注释进行泛基因组分析.

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