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Tissue Homogenization and Cell Lysis01:32

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Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
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相关实验视频

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Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
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针对心脏组织脱细胞化的基于单一洗剂的协议的优化技术

Mihai Meșină1, Ion Mîndrilă2, Mihaela Iustina Meșină-Botoran2

  • 1Doctoral school, University of Medicine and Pharmacy of Craiova, Romania.

Current health sciences journal
|October 2, 2023
PubMed
概括

脱氧化 (SD) 有效地去细胞化心脏组织,用于再生医学支架. 优化SD协议,包括透冲击和洗时间,增强细胞外矩阵 (ECM) 制造用于组织修复.

关键词:
这是心脏ECM.原蛋白纤维 原蛋白纤维脱细胞化 脱细胞化脱氧化酸是一种脱氧化.硫酸 Lauryl 硫酸是一种

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科学领域:

  • 生物材料科学 生物材料科学
  • 再生医学是一种再生医学.
  • 组织工程是组织工程.

背景情况:

  • 细胞外基质 (ECM) 支架对于再生医学和组织修复至关重要.
  • 心肌组织的脱细胞化是获得自然ECM移植的关键过程.
  • 优化脱细胞化参数对于有效的心脏ECM制造至关重要.

研究的目的:

  • 进行心脏组织脱细胞化的形态和形态测量分析.
  • 为了比较基于脱氧化 (SD) 和酸 (SLS) 的协议.
  • 确定优化单一洗剂心脏ECM生产的因素.

主要方法:

  • 维斯塔老鼠心脏 (n=10) 接受了5种不同的脱细胞化方案 (n=2).
  • 组织学处理包括血素和乙素 (H&E) 和Azan三色染色.
  • 对所得到的ECM进行了形态和形态测量分析.

主要成果:

  • 发现硫酸 (SLS) 可以改变心脏ECM原纤维的空间分布.
  • 脱氧化酸 (SD) 允许成功地定制单一洗剂协议.
  • 调整低/高度冲击和洗时间的应用时间可以增强SD的化作用和残留物消除.

结论:

  • 脱氧化酸 (SD) 是优化单一洗剂心脏ECM制造的一种可行的药物.
  • 仔细调整透冲击和洗时间对于使用SD的有效脱细胞化至关重要.
  • 优化的SD协议可以为再生医学应用提供高质量的心脏ECM支架.