通过基修饰与基结合域进行LL37与原蛋白结合的表征
Ziqi Wei1, Marsha W Rolle2, Terri A Camesano1
1Department of Chemical Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester, Massachusetts 01609, United States.
ACS omega
|October 2, 2023
概括
我们研究了修改后的抗微生物 (AMP) 如何与原结合,以获得更好的伤口愈合. 原结合域 (cCBD) 提高了的稳定性和保持在原支架上,这表明改善了伤口护理应用.
科学领域:
- 生物材料科学 生物材料科学
- 伤口愈合 治愈 伤口愈合
- 抗微生物是一种抗菌.
背景情况:
- 与抗微生物 (AMP) 结合的原基生物材料对伤口愈合和感染控制具有前景.
- 之前的工作修改了AMP LL37以原结合域 (cCBD) 来改善原料的保留.
- 修改了cCBD的LL37 (cCBD-LL37) 与原的结合机制需要进一步阐明.
研究的目的:
- 为了阐明cCBD-LL37与原的结合机制.
- 调查原结合域 (cCBD) 在-相互作用中的作用.
- 评估cCBD对的结构稳定性和保留的影响.
主要方法:
- 亲和度测量比较LL37和cCBD-LL37与原蛋白结合.
- 使用不同离子强度和pH的静电相互作用研究.
- 循环二重化谱学分析的结构稳定性.
- 从原体支架释放的评估.
主要成果:
- cCBD-LL37对原的亲和力略高于LL37.
- cCBD部分抑制,但没有消除cCBD-LL37结合,这表明多部位相互作用.
- 这两种都通过静电力与原相互作用,过渡到近距离或疏水性相互作用.
- 与LL37相比,cCBD-LL37在不同的条件下表现出更强的结构稳定性.
- cCBD在原支架上增强了的保留,尽管释放受到静电环境的影响.
结论:
- cCBD-LL37与原的结合涉及通过cCBD和非特异力进行的特定相互作用.
- 该cCBD部分增强了LL37在原体支架上的结构稳定性和保留.
- 这些发现提供了对优化基于原的伤口包裹与修改AMPs改善伤口护理的见解.
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