相关实验视频
Updated: Jul 15, 2025
09:00
Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
Published on: July 27, 2013
18.6K
基于库马林的简单光探针用于识别Pd (II) 和其活细胞成像
Xiangyang Zhang1,2, Yaodan Xu1,2, Youming Shen1,2
1College of Chemistry and Material Engineering, Hunan University of Arts and Science, Changde 415000, P. R. China.
ACS omega
|October 2, 2023
概括
一个新的光探测器,库马林氨酸希夫基 (CBBS),可以选择性地检测高灵敏度的Palladium (II) 离子. 这种探测器有效用于在活细胞中成像Palladium (II) 并开发用于其检测和吸附的聚合物.
科学领域:
- 分析化学 分析化学
- 化学传感器 化学传感器
- 材料科学 材料科学 材料科学
背景情况:
- 是催化和生物系统中的关键元素.
- 对于Palladium的选择性和敏感的检测方法是必不可少的.
- 光探头在灵敏度和实时监控方面具有优势.
研究的目的:
- 设计和合成一种新型库马林化 Schiff基 (CBBS) 探针.
- 为了评估探测器的选择性和敏感性,以检测.
- 为了展示探针在活细胞中的光成像中的应用.
主要方法:
- 理性的设计和简单的合成CBBS探针.
- 用光谱分析 (光) 来确定检测极限和线性.
- 光成像探针在A549细胞中的应用.
主要成果:
- CBBS对Pd (II) 具有很好的选择性,其低检测极限为65nM.
- 光强度显示对Pd (II) 度从0到80微米的线性反应.
- 在活体A549细胞中成功实现了Pd(II) 的光成像.
结论:
- CBBS是一种高度选择性和敏感的光探针,用于Pd.
- 该探测器适用于生物系统中Pd(II) 的实时光成像.
- CBBS为开发用于Pd (II) 检测和吸附的聚合物提供了基础.
相关概念视频
03:31Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
01:19Protein Dynamics in Living Cells
2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K