大豆蛋白与多的相互作用:自然和化形式的结构和功能变化
Shizhang Yan1, Qi Wang1, Jiaye Yu1
1College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Food chemistry: X
|October 2, 2023
概括
将大豆蛋白分离物 (SPI) 与乙烯基胺 (EDA) 接种,产生了阴性大豆蛋白 (NSPI),增强了其乳化和抗氧化特性. 这些修改后的蛋白质与酸 (GA) 形成复合体,改善了潜在的食品应用的功能.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物材料是一种生物材料.
背景情况:
- 豆蛋白分离物 (SPI) 是一种具有功能性质的常见食品成分.
- 修改SPI可以提高其作为乳化剂和抗氧化剂的性能.
- 已知乙二胺 (EDA) 和酸 (GA) 与蛋白质相互作用.
研究的目的:
- 通过将EDA接种到SPI上来合成阴阳性大豆蛋白分离物 (NSPI).
- 为了形成和表征NSPI-酸 (GA) 复合体.
- 评估NSPI-GA复合对蛋白质功能,包括乳化和抗氧化能力的影响.
主要方法:
- 通过EDA在SPI上接种NSPI的合成.
- 在不同比例下形成NSPI-GA复合体.
- 结构性,热性,颗粒大小,乳化和抗氧化性质的表征.
- 使用光谱方法分析蛋白质-连接物相互作用.
主要成果:
- EDA接种给SPI传递了正电荷,产生了具有均颗粒大小的NSPI.
- 与SPI相比,NSPI表现出增强的热稳定性,乳化能力和抗氧化能力.
- NSPI显示氨基基群增加和与GA更强的相互作用.
- EDA和GA协同增加了蛋白质的灵活性,改变了二次结构 (减少α螺旋,增加随机线圈).
- 相互作用被确定为静态,疏水性 (GA-SPI) 和静电性 (GA-NSPI).
结论:
- 使用EDA移植SPI显著改善其功能性质.
- NSPI-GA复合体表现出增强的乳化和抗氧化能力.
- 修改后的蛋白质连接体复合体具有作为食品系统中功能成分的潜力.
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