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Updated: Jul 15, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

单核酸分辨率绘制基因组DNA中的N6甲基氨酸

Cheng-Jie Ma^1,2,3, Gaojie Li^4,5, Wen-Xuan Shao^1,2

¹School of Public Health, Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China.

ACS central science

|October 2, 2023

概括

我们开发了DM-seq,这是绘制全基因组N6-Methyladenine (6mA) DNA修改图的新方法. 这项技术精确地识别了细菌和人类线粒体DNA中的6mA位点,有助于疾病研究.

科学领域:

表观遗传学和基因组学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

N6-Methyladenine (6mA) 是一种在各种生物体中发现的显著DNA修饰.
了解6mA分布对于破译其生物作用至关重要.
现有的方法可能缺乏全面的6mA映射的分辨率或特异性.

研究的目的:

开发和验证一种用于以单核酸分辨率进行全基因组6mA映射的新方法.
将这种方法应用于研究 prokaryotic (大肠杆菌) 和eukaryotic (哺乳动物mtDNA) 系统中的6mA分布.
探索6mA在线粒体相关人类疾病中的潜力.

主要方法:

开发了使用进化腺因脱氨酶 (ABE8e) 的脱氨酶介导测序 (DM-seq).
在保持6mA的同时,选择性去除腺因,以实现精确的检测.
在大肠杆菌和人类HepG2细胞mtDNA中应用DM-seq进行全基因组6mA分析.

主要成果:

在单核酸分辨率下成功地在大肠杆菌中绘制了6mA的全基因组映射.
在大肠杆菌基因组中,主要在GATC基因内识别6mA位点.
在HepG2细胞mtDNA中发现了17个6mA位点,仅在重链上.

结论:

DM-seq为高分辨率的全基因组6mA检测提供了一个强大的工具.
这些发现揭示了细菌和人类线粒体中6mA分布的特定模式.
对于6mA功能及其与线粒体疾病的联系,DM-seq对未来的研究充满希望.

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DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

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