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在食道状细胞癌中,circ_0004365和西斯普拉丁耐药性之间存在关联

Moyuru Yamada¹, Koji Tanaka¹, Kenichi Yamamoto²

¹Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.

Oncology letters

|October 2, 2023

概括

循环RNA (circRNA) circ_0004365在食道状细胞癌 (ESCC) 中是上调调的,并且与西斯普拉丁耐药性有关. 这一发现表明circ_0004365作为ESCC化疗的潜在生物标志物和治疗标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

西斯普拉丁是食道状细胞癌 (ESCC) 的主要化疗方法.
对于ESCC中西斯普拉丁耐药性的机制在很大程度上是未知的.
循环RNAs (circRNAs) 正在成为各种癌症的关键调节者.

研究的目的:

为了研究circRNAs在ESCC中对西斯普拉丁耐药性的作用.
确定潜在的circRNA生物标志物,以预测ESCC患者的治疗反应.

主要方法:

在ESCC细胞 (TE11) 和抗细胞 (TE11R) 中的circRNAs的RNA测序.
CIRCexplorer2用于表达方式分析.
反转录量PCR (RT-qPCR) 用于验证.
测量MTT测试以评估circRNA淘汰对西斯普拉丁耐药性的影响.

主要成果:

Circ_0004365,映射到Semaphorin 3C基因中,与TE11细胞相比,TE11R细胞的表达显著更高.
在ESCC瘤组织中,Circ_0004365的上调调节,特别是在化疗后.
升高的circ_0004365表达与ESCC患者的病理反应不佳和晚期PT阶段相关.
在TE11细胞中击败circ_0004365降低了对西斯的抗性.

结论:

在ESCC中,Circ_0004365与西斯普拉丁耐药性有关.
Circ_0004365可以作为一种新的生物标志物,用于预测ESCC的治疗结果.
Circ_0004365代表了在ESCC中克服西斯普拉丁耐药性的潜在治疗标.

关键词:

在CIRC探索者.通过RNA测序进行RNA测序.圆形的RNARNA是一个圆形的RNA.西斯普拉丁 (cisplatin) 是一种制剂.食管状细胞癌的癌症

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