不同提取方法对固态发酵咖啡提取物生物活性化合物成分和抗氧化特性变化的影响
Rossaporn Jiamjariyatam1, Pakkapong Phucharoenrak2, Siritron Samosorn1
1Department of Chemistry, Faculty of Science, Srinakharinwirot University, 114 Sukhumvit 23, Bangkok 10110, Thailand.
TheScientificWorldJournal
|October 2, 2023
概括
咖啡是一种食物浪费,可以发酵并提取有价值的生物活性化合物. 在60°C的乙醇提取产生了最高的甘和抗氧化活性,展示了可持续的潜力.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 可持续化学 可持续化学
背景情况:
- 咖啡造产生大量的食物浪费,主要是咖啡.
- 咖啡含有丰富的生物活性化合物,如咖啡因,酸和氨酸.
- 为了可持续的资源利用,需要对咖啡进行全面利用.
研究的目的:
- 研究提取方法对发酵咖啡生物活性化合物和抗氧化活性的影响.
- 评估咖啡作为有价值提取物的来源的潜力.
主要方法:
- 使用*Saccharomyces cerevisiae**进行咖啡的固体发酵.
- 在不同温度 (60°C,100°C) 中以乙醇或水提取.
- 使用Folin-Ciocalteu测定和H-NMR光谱法对生物活性化合物的量化.
- 通过DPPH和FRAP测定评估抗氧化活性.
主要成果:
- 提取产量在100°C时最高.
- 在60°C发酵的的乙醇提取物显示出最高的生物活性含量 (3.59%的trigonelline) 和抗氧化活性 (23.35% DPPH,25.9% FRAP).
- 生物活性含量和抗氧化剂活性受原料,制备和提取条件的影响.
结论:
- 发酵咖啡是一种有价值的,可持续的生物活性化合物和抗氧化剂提取物的来源.
- 优化提取方法对于最大限度地回收有益化合物至关重要.
- 这项研究突出了将咖啡皮废弃物价值化为高价值产品的潜力.
相关概念视频
Oxidations of Aldehydes and Ketones to Carboxylic Acids
Oxidation of aldehydes and ketones results in the formation of carboxylic acids. Aldehydes, bearing hydrogen next to the carbonyl group, are easily oxidized compared to ketones. This is because an aldehydic proton can easily be abstracted during oxidation.
Aldehydes readily undergo oxidation in strong oxidizing agents such as potassium permanganate and chromic acid. The oxidation can also be carried out using mild oxidizing agents such as silver oxide. In fact, aldehydes can be easily oxidized...
Aldehydes readily undergo oxidation in strong oxidizing agents such as potassium permanganate and chromic acid. The oxidation can also be carried out using mild oxidizing agents such as silver oxide. In fact, aldehydes can be easily oxidized...
Radical Autoxidation
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
Extraction: Effects of pH
Consider a neutral form of an amine, B, with a partition coefficient, K, in a liquid mixture containing organic and aqueous phases. The pH of the aqueous phase affects the charge on acidic and basic solutes, and the charged form is usually more soluble in the aqueous phase. Suppose the conjugate acid form of the amine is soluble only in the aqueous phase while the base form is soluble in both phases. Then the distribution coefficient, D, can be given as the ratio of amine concentration in the...
Extraction: Advanced Methods
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...


