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Updated: Jul 15, 2025

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

在hsa-miR-3117-3p的成熟序列中的新型多态 rs12402181通过影响miRNA处理和功能,对乳腺癌的发展产生保护作用

Razieh Mirabbasi¹, Seyed Omar Ebrahimi², Fatemeh Tavakoli²

¹Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

|October 2, 2023

概括

在miR-3117-3p的一个遗传变异 (rs12402181) 与降低乳腺癌风险有关. 这种变异增加了成熟的microRNA产量,抑制了癌细胞的增殖和迁移.

科学领域:

遗传学是一个遗传学.
分子生物学分子生物学
在瘤学瘤学.

背景情况:

微RNA (miRNA) 在基因调节中起着至关重要的作用,并与包括乳腺癌在内的各种癌症有关.
在miRNA基因或它们的调节区域内的遗传变异可以影响miRNA生物发生,功能和疾病易感性.

研究的目的:

为了研究rs12402181单核酸多态 (SNP) 在miR-3117-3p种子区域的功能影响.
为了确定rs12402181多态和乳腺癌易感性之间的关联.
评估变异对miRNA处理,标结合和癌细胞行为的影响.

主要方法:

在乳腺癌患者和使用PCR-RFLP的健康对照中rs12402181多态的基因定型.
在体外分析涉及表达载体,以评估SNP对miR-3117-3p表达水平和功能的影响.
细胞增殖和迁移测定 (MTT和伤口愈合) 来评估功能后果.
生物信息工具用于预测miRNA功能的变化.

主要成果:

rs12402181的多态性 (G>A) 显示出与乳腺癌易感性的反向关联 (P=0.03,OR=0.551).
变异性等位基因 (A) 导致成熟的miR-3117-3p的产生增加.
这种增加的成熟miRNA水平导致乳腺癌细胞系 (MCF7和T47D) 的细胞增殖和迁移减少.

结论:

在miR-3117-3p中的rs12402181变体影响了miRNA处理,增强了成熟的miRNA形式.
这种增强成熟的miR-3117-3p作为瘤抑制剂,负面调节乳腺癌的发展和迁移.
rs12402181的多态性代表了一种潜在的保护性遗传因子,可以预防乳腺癌.

关键词:

乳腺癌乳腺癌乳腺癌多态化多态化.在 miR-3117-3pp 中.在 miRNA 处理过程中.

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