两种不同的埃及传染性支气管炎菌株的病理评估和组织热带性
El-Shaymaa El-Nahass1, Mohamed Kamal Abdelhamid1, Ahmed Ali2
1Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511 Egypt.
Virusdisease
|October 2, 2023
概括
经典和变异性传染性支气管炎病毒 (IBV) 菌株在中表现出类似的轻微影响. 感染IBV可能导致免疫抑制,现场死亡率可能是由于二次感染.
科学领域:
- 兽医病毒学 兽医病毒学
- 禽类疾病 禽类疾病
- 免疫学 免疫学 免疫学
背景情况:
- 禽传染性支气管炎病毒 (IBV) 在家禽养殖中带来了重大挑战,因为病毒株正在演变.
- 了解不同IBV菌株的热带性和致病性对于有效的疾病控制至关重要.
研究的目的:
- 在特定无病原体 (SPF) 中比较经典和变异IBV菌株的病原性和组织热带性.
- 调查IBV感染引起的免疫抑制的可能性.
主要方法:
- SPF小被用经典或变种IBV菌株进行了鼻腔接种.
- 在感染后的15天内,对临床症状,死亡率和严重病变进行了监测.
- 组织病理学,免疫组织化学 (IHC) 和定量逆转录聚合酶连锁反应 (qRT-PCR) 用于病毒检测和组织分析.
主要成果:
- 这两种IBV菌株都引起了轻微的临床症状,没有死亡率或显著的严重病变.
- 病毒RNA被qRT-PCR检测到感染后12天,但病毒抗原没有被IHC检测到感染后10天.
- 感染后10天观察到组织再生,在毛囊中检测到IBV抗原,这表明潜在的免疫抑制.
结论:
- 在SPF的经典和变体IBV菌株的行为中没有发现显著差异.
- 广泛的组织热带和缺乏严重的病变表明,IBV的现场死亡率通常是由于二次感染.
- 需要进一步的研究来证实IBV感染的免疫抑制作用.
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