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微塑料和纳米塑料的分离和流动细胞计分析

Jingjing Li¹, Fuyi Huang^1,2, Guohui Zhang^1,3

¹Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China.

Frontiers in chemistry

|October 2, 2023

概括

这项研究增强了流量细胞计,用于使用尼罗河红色染色来检测微塑料和纳米塑料. 优化的协议提高了环境分析的准确性和更低的检测极限.

科学领域:

环境科学环境科学
分析化学分析化学
毒理学毒理学毒理学

背景情况:

流细胞计越来越多地用于微塑料分析.
目前的方法需要改进,以准确地计数微塑料和纳米塑料.
尼罗河红色 (NR) 染色是一种常见的技术,但其效率可以提高.

研究的目的:

优化流量细胞测量协议,以便更好地检测尼罗河红色染色的微塑料和纳米塑料.
提高微塑料和纳米塑料量化的准确性和灵敏性.
为评估微塑料和纳米塑料污染提供一种可靠的方法.

主要方法:

通过调整二甲基硫氧化物 (DMSO) 度,优化了尼罗河红色染色协议.
使用特定的点图 (侧散与黄色光) 进行准确的计数.
确定最佳NR染色度 (15-20μg/mL) 和流细胞计参数.
采用顺序过用于微塑料和纳米塑料的分离和度.
在11种微塑料类型和模拟水样中验证了该方法.

主要成果:

增加DMSO度 (20%-30%) 改善了NR的溶解性,并减少了聚合.
最佳NR染色度确定为15-20μg/mL.
微塑料检测的下限为10^4颗粒/毫升,最佳范围为10^5-10^6颗粒/毫升.
流式细胞计的最小可检测颗粒大小为150nm.
过和流量细胞计方法在模拟样本中显示了微塑料和纳米塑料的高回收率 (0.80-1.19).

结论:

增强的流量细胞测量协议提供了微塑料和纳米塑料的准确和敏感量化.
优化的方法对各种微塑料类型和环境矩阵有效.
这种方法为微塑料和纳米塑料毒性评估提供了关键的技术支持.

关键词:

尼罗河红色尼罗河红色计数地区地区计数地区.流动细胞计量是流动细胞计量的方法.微塑料微塑料的使用多个阶段的过过.纳米塑料是一种纳米塑料.

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