相关实验视频

Updated: Jul 15, 2025

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques

Published on: March 24, 2016

用Enterobacter hormaechei KS1 Xylanase使用小麦生产的西洛利戈糖的生产,特征和益生菌潜力

Kajal Kumari¹, Sushil Nagar¹, Sakshi Goyal¹

¹Department of Biochemistry, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, Hisar, Haryana 125004 India.

Indian journal of microbiology

|October 2, 2023

概括

来自Enterobacter hormaechei KS1的新型西兰酶可以有效地从小麦和西兰中产生西. 这种酶性水解产生有价值的化合物,可用于潜在的益生菌应用.

科学领域:

生物技术是生物技术.
食品科学食品科学食品科学
微生物学微生物学

背景情况:

小麦是一种丰富的农业副产品,富含西兰.
氧氧糖 (Xylooligosaccharides,XOS) 是一种具有潜在前生物特性的功能性糖.
酶性水解为生产XOS提供了一种可持续的方法.

研究的目的:

通过使用来自Enterobacter hormaechei KS1.1.的新型西兰酶,从小麦和提取的西兰中制造氧化氧化糖 (XOS).
为了优化酶解水解条件以获得最大的XOS产量.
评估产生的XOS对所选乳酸细菌的pH值和光密度的影响.

主要方法:

用Enterobacter hormaechei KS1 xylanase进行小麦和提取的西兰的酶性水解.
优化反应参数,包括基质度,酶剂量,pH,温度和化时间.
使用高性能液态染色学 (HPLC) 分析水解盐成分.
评估XOS对Lactobacillus物种pH和光学密度的影响.

主要成果:

在优化条件下,小麦的最大XOS产量为374mg/g (4.0%小麦,375IU/mL酶,pH6.0,50°C,24h).
从提取的西兰中最大的XOS产量为419 mg/g (3%西兰,8小时化).
在HPLC分析中证实了水解酸中含有西洛,西洛和西洛赫索的存在.
从提取的西兰中提取的XOS显著降低了Lactobacillus rhamnosus,L. brevis和L. plantarum培养中的pH值.
在这些细菌中,XOS增加了光学密度,其中L. rhamnosus表现出最高的响应.

结论:

肠杆菌hormaechei KS1氧化酶是有效的生产XOS从小麦和西兰.
优化的酶解水解可以产生大量具有潜在益生菌活性的XOS.
生产的XOS可以影响有益的肠道细菌的生长和代谢活动.

关键词:

牛乳杆菌 (Lactobacillus) 是一种细菌.小麦干的小麦干的小麦干.提取的小麦提取了西兰.在Xylanase的使用中.氧化氧糖化物 (Xylooligosaccharides) 是一种氧化.

更多相关视频

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

相关概念视频

Biosynthesis of Polysaccharides

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...