路边颗粒物和对SARS-CoV-2感染的易感性
Lisa Miyashita1, Gary Foley1, Sean Semple2
1Blizard Institute, Queen Mary University of London, London, United Kingdom.
The journal of allergy and clinical immunology. Global
|October 2, 2023
概括
暴露于颗粒物 (PM10) 会增加血管素转化酶2 (ACE2) 的表达,增加了实验室细胞模型中对严重急性呼吸综合征冠状病毒2型 (SARS-CoV-2) 感染的易感性. 这一发现提供了一个潜在的生物机制,将空气污染与COVID-19的严重程度联系起来.
科学领域:
- 环境健康 环境健康
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 颗粒物 (PM10) 和COVID-19严重程度之间的关联缺乏确定的生物可信性.
- 微小可能上调血管素转化酶2 (ACE2),SARS-CoV-2入口受体,作为一个潜在的机制.
研究的目的:
- 为了研究PM10对SARS-CoV-2感染细胞in vitro的影响.
- 探索ACE2上调的作用作为一个机制.
主要方法:
- 培养了各种人类细胞系 (A549,HPNEpCs,Calu3) 和SARS-CoV-2-敏感的Vero-E6细胞,其中PM10来自路边和排气排放.
- 通过流细胞计和PCR评估ACE2表达和mRNA水平.
- 在Vero-E6细胞中评估了SARS-CoV-2的细胞病变效应和病毒基因表达.
- 使用N-乙半氨酸研究了氧化应激的作用.
主要成果:
- 暴露于PM10显著增加了A549,HPNEpCs和Calu3细胞中的ACE2表达.
- 在HPNEpCs中,N-乙囊减弱的PM10诱导了ACE2表达,这表明氧化应激的作用.
- 在Vero-E6细胞中,PM10增加了ACE2表达,ACE2转录水平,SARS-CoV-2细胞病变效应和病毒基因表达.
结论:
- 路边的PM10暴露增强了ACE2的表达,并增加了对SARS-CoV-2感染的敏感性.
- 这项研究提供了一个潜在的生物机制,将空气污染与COVID-19风险增加联系起来.
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