在甲固定心肌中自光的管理:选择正确的治疗方法
Zhao Zhang1, Hongming Fan2, William Richardson3
1Department of Bioengineering, Clemson University, Clemson, SC. zz2@clemson.edu.
European journal of histochemistry : EJH
|October 2, 2023
概括
心脏组织中的自发光 (AF) 阻碍了免疫光 (IF) 成像. 苏丹黑B和TrueBlack有效地抑制AF,SBB显示出优越的火,TrueBlack最好地保留IF信号.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 历史学 历史学 历史学
- 细胞生物学 细胞生物学
背景情况:
- 像心肌这样的组织中的自光 (AF) 干扰了使用免疫光 (IF) 显微镜检测特定蛋白质.
- 现有的AF抑制方法缺乏对心肌样本的彻底验证,在不同的自光结构中可能存在不一致.
- 有效的AF火对于在具有挑战性的组织中进行准确的现场蛋白质分析至关重要.
研究的目的:
- 量化评估和比较常见的AF火处理在甲固定心肌样本上的疗效.
- 在不同的免疫光染色条件下 (有或没有洗剂) 评估这些处理的性能.
- 确定AF抑制对自发光结构和特定的IF标签的影响.
主要方法:
- 用甲固定心肌样本被各种AF灭剂处理:甘氨酸,苏丹黑B (SBB),玻化 (NaBH4),TrueVIEW®和TrueBlack®.
- 使用染色前和染色后协议进行处理,以模拟不同的IF工作流情景.
- 灭效率被定量评估,并对主要的自光心肌结构和IF信号完整性的影响进行了评估.
主要成果:
- 苏丹黑B (SBB) 和TrueBlack®在两种测试方案中,与其他药物相比,表现出优异的AF抑制.
- SBB可以更好地灭主要的自光心肌结构,而TrueBlack®在保存IF标记信号方面表现出色.
- 化 (NaBH4) 意外地增强了AF信号和对比度,这表明它有可能作为肌动脉结构可视化的AF增强剂.
结论:
- SBB和TrueBlack®是有效的试剂,用于抑制甲固定心肌样本中的自光.
- 在SBB和TrueBlack®之间做出选择取决于是否优先考虑最大AF灭或IF信号的保存.
- NaBH4表现出一个意想不到的作用,作为一个自光增强剂,可能有助于心肌结构的识别,而无需对抗染色.
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