通过配对的效应体促进交感效应体蛋白移动性在不同类型的真菌病原体中得到保护
Nacera Talbi1, Mila C Blekemolen2, Slavica Janevska2
1Université Paris-Saclay, INRAE, UR BIOGER, 91120 Palaiseau, France.
Molecular plant-microbe interactions : MPMI
|October 2, 2023
概括
植物病原体使用效应蛋白通过细胞体 (PD) 在细胞之间传播. 这项研究表明,不同真菌中相关的效应因子对操纵PD进行细胞间移动,帮助感染.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 细胞生物学 细胞生物学
背景情况:
- 植物病原体分泌效应物来操纵宿主细胞.
- 等离子体 (PD) 是植物细胞间通信的通道.
- Fusarium oxysporum (Fol) 使用Six5/Avr2效应器对扩展PD,使效应器运动到未感染的细胞.
研究的目的:
- 调查其他真菌病原体是否利用类似的效应器对来操纵PD.
- 为了确定不同真菌物种Six5/Avr2类效应器对的结构和功能保存.
主要方法:
- 来自Leptosphaeria maculans (Lm) 的效应体对的结构预测.
- 在PDLm因子的表达和局部化研究.
- 功能补充测定使用Fol Six5淘汰突变的功能补充测定.
主要成果:
- Lm AvrLm10A同类在结构上与Fol Six5相似,并定位在PD.
- 这些lm效应器可以补充Fol Six5突变的细胞间移动缺陷.
- Lm效应器恢复了Fol Six5淘汰突变体中的毒性.
结论:
- 遥远相关的真菌病原体使用结构保守的效应因子对来操纵PD.
- 六五介导的效应因子转位是真菌植物病原体中保留的一种机制.
- 这种保存的机制促进了效应体的移动性和宿主感染.
关键词:
三维结构的3D结构.这里是 Fusariumarium .莱普托斯法里亚菌 (Leptosphaeria) 是一种从细胞到细胞的移动性.效应器对的效应器对.菌是一种真菌.植物病原体 植物病原体塑体可能会发生变化.更多相关视频
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