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拉利通过触发Eif4g2基因表达在初级感觉神经元中,参与神经创伤诱导的感知过敏

Lina Huang¹, Dilip Sharma¹, Xiaozhou Feng¹

¹Department of Anesthesiology, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, New Jersey, USA.

British journal of pharmacology

|October 2, 2023

概括

在神经受伤后,RNA结合蛋白RALY通过增加Eif4g2基因转录在背部根结质神经元中来驱动神经病痛. 抑制RALY可能为控制神经病痛提供了一个新的策略.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
疼痛研究疼痛研究

背景情况:

周围神经创伤会改变背部根结质 (DRG) 神经元中的基因表达,导致神经病痛.
RNA结合蛋白在调节基因转录中起着至关重要的作用.

研究的目的:

研究RNA结合蛋白RALY在神经创伤引起的基因失调和神经病痛中的作用.
为了确定RALY是否影响DRG神经元中与疼痛相关的基因的表达.

主要方法:

免疫组织化学检测DRG神经元中的RALY表达.
慢性收缩损伤 (CCI) 模型的外周神经创伤.
对Raly shRNA和Raly mRNA向DRG传递的阿诺相关病毒5 (AAV5) 载体.
对Raly和Eif4g2mRNA和蛋白质水平的量化.
对 nociceptive 过敏和运动运动活动的评估.

主要成果:

在CCI之后,DRG神经元的RALY表达增加.
镇压RALY减少了CCI诱导的Eif4g2表达和神经病痛.
在天真的DRG神经元中RALY的过度表达模仿了疼痛过敏和增加Eif4g2水平.
发现RALY与Eif4g2基因促进体结合,增强其转录活性.

结论:

拉利在调解神经创伤引起的神经病痛方面发挥着至关重要的作用.
RALY在转录上调节了DRG神经元中的Eif4g2表达.
拉利代表了神经性疼痛管理的潜在治疗标.

关键词:

如果4g2 拉力会是什么意思背部根系团的结节.神经创伤的神经创伤神经病变性疼痛是一种神经性疼痛.

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