对患有喉回流性疾病的患者的喉微生物群的分析
1The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Department of Otolaryngology, Yiwu, China.
Brazilian journal of otorhinolaryngology
|October 2, 2023
概括
质子抑制剂治疗改变了喉回流病 (LPRD) 患者的喉微生物组. 克莱布西拉氧托卡 (Klebsiella oxytoca) 丰富度可以预测治疗的有效性,为LPRD结果提供潜在的生物标志物.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
背景情况:
- 喉回流性疾病 (LPRD) 通常使用质子抑制剂 (PPI) 治疗.
- 在LPRD患者中,PPI治疗对喉喉微生物群的影响尚未完全理解.
- 识别生物标志物来预测LPRD治疗反应对于个性化医学至关重要.
研究的目的:
- 在LPRD患者中,在PPI治疗后,对喉喉微生物组的改变进行鉴定.
- 将LPRD患者的微生物组与健康对照组进行比较.
- 探索Klebsiella oxytoca作为LPRD治疗的潜在结果预测生物标志物.
主要方法:
- 在LPRD患者和健康对照人群中,使用盘子计数和PCR-DGGE分析了喉喉微生物组组成.
- 使用香农-维纳和均度指数评估了细菌多样性.
- 滴滴数字PCR量化了总细菌RNA和Klebsiella oxytoca的丰度;ROC曲线评估了Klebsiella oxytoca作为生物标志物.
主要成果:
- 微生物组的组成在两组之间是相似的,在Klebsiella oxytoca.有显著的差异.
- 微生物群的多样性和丰富性在健康,治疗有效,预治疗和治疗无效的LPRD群体之间存在差异.
- 与治疗无效组相比,在治疗有效的LPRD组中观察到较低的Klebsiella oxytoca丰度 (AUC=0.859).
结论:
- 喉喉微生物群的多样性在治疗无效和治疗有效的LPRD群体之间存在差异.
- 克莱布西拉氧托卡 (Klebsiella oxytoca) 的丰富性显示出作为区分LPRD治疗结果的生物标志物的潜力.
- 这些发现可能有助于预测患者对PPI治疗LPRD的反应.
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