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开发一种表观遗传年龄预测器,用于肋骨软骨,同时具有体质组织分化系统

A Freire-Aradas¹, M Tomsia², D Piniewska-Róg³

¹Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Spain.

Forensic science international. Genetics

|October 2, 2023

概括

法医科学家现在可以使用DNA甲基化来从软骨DNA中预测年龄. 这项研究还开发了一种模型,使用DNA甲基化标记物来区分血液,口腔细胞,骨和软骨.

科学领域:

法医科学法医科学法医科学
遗传学是一个遗传学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

随着年龄的增长,DNA甲基化模式发生变化,从而使表观遗传年龄的估计成为可能.
现有的年龄预测模型通常是组织特异性的,需要不同的生物样本单独的模型.
VISAGE增强工具同时分析多个与年龄相关的DNA甲基化位点.

研究的目的:

利用DNA甲基化数据开发一种用于软骨的法医年龄预测模型.
评估 VISAGE 增强工具标记物在区分各种人体组织中的实用性.
扩大DNA甲基化分析在法医调查中的应用.

主要方法:

开发了一个软骨年龄预测模型,使用多变量定量回归对来自三个CpG (FHL2,TRIM59,KLF14) 的DNA甲基化数据进行了多变量回归.
使用了109个软骨样本的训练组和72个样本的独立测试组.
使用基于8个VISAGE标记的多项逻辑回归创建了一个组织分化模型 (EDARADD,TRIM59,ELOVL2,MIR29B2CHG,PDE4C,ASPA,FHL2,KLF14).

主要成果:

软骨年龄预测模型在训练组中实现了平均绝对误差 (MAE) ±4.41年,在测试组中达到±4.26年.
组织分化模型准确地分类了血液,口腔细胞,骨和软骨,具有高灵敏度和特异性.
培训组获得了98.72%的正确分类,而测试组获得了97.4%的正确分类.

结论:

已经建立了一个强大的法医软骨年龄预测模型,增强了死后样本的年龄估计.
8个VISAGE标记器有效地区分关键的法医组织,增加单个分析的信息产量.
这两种开发的模型都可在Snipper法医分类网站上找到,以支持法医案例工作.

关键词:

通过DNA甲基化.年龄估计年龄估计.血液血液血液血液血液骨头骨头骨头骨头嘴巴细胞是什么?肋骨软骨肋骨软骨是一种组织识别组织识别

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